Effects of green tea extracts on gene expression in HepG2 and Cal-27 cells.
نویسندگان
چکیده
Green tea extract is known to contain compounds that are able to produce antioxidant effects in many types of living cells. Treatment of cultured human hepatoma (HepG2) cells with green tea extract resulted in dramatically increased expression of at least 15 genes that are present on a commercial human drug metabolism gene array. RT-PCR was used to confirm the microarray results, and analysis of the 5'-flanking region of each of these genes revealed potential electrophile/antioxidant response elements. Members of the acetyl transferase, epoxide hydrolase, sulfotransferase and glutathione transferase gene families were strongly induced. In addition, the human tongue carcinoma cell line Cal-27 did not respond to green tea extract in the same way, as none of the induced genes in the HepG2 cells were induced in the Cal-27 cells. The lack of induction of detoxification enzymes in the Cal-27 cell line may help to explain the previously observed increased cytotoxicity of green tea catechins on this cell line.
منابع مشابه
Cytochrome P450 expression and activities in human tongue cells and their modulation by green tea extract.
The expression, inducibility, and activities of several cytochrome P450 (CYP) enzymes were investigated in a human tongue carcinoma cell model, CAL 27, and compared with the human liver model HepG2 cells. The modulation effects of green tea on various CYP isoforms in both cell lines were also examined. RT-PCR analysis of CAL 27 cells demonstrated constitutive expression of mRNA for CYPs 1A1, 1A...
متن کاملBoth Phenolic and Non-phenolic Green Tea Fractions Inhibit Migration of Cancer Cells
Green tea consumption is associated with chemoprevention of many cancer types. Fresh tea leaves are rich in polyphenolic catechins, which can constitute up to 30% of the dry leaf weight. While the polyphenols of green tea have been well investigated, it is still largely unknown, whether or not non-phenolic constituents also reveal chemopreventive and anti-metastatic effects. In this study, we i...
متن کاملJujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production
Objective: Oxidative stress and ultraviolet B (UVB) irradiation are known as principal inducers of DNA damage and modulators of gene expression in aging process and skin photoaging, which are associated with upregulation of matrix metalloproteinases (MMPs). Because of the antioxidant capacity of jujube and green tea, we decided to determine their protective effects of human fib...
متن کاملEffect of Eight Weeks Aerobic Exercise and Green Tea on Atherogenic Ratio and ABCG8 Gene Expression of PBMC Globules in Overweight Women
Objective: ABCG8 plays a crucial role in the reverse transfer of cholesterol from macrophages to the liver. The herbal compliments and regular physical improve the lipid profile and metabolic disorders. The aim of this study was to evaluate the effect of eight weeks aerobic exercise and green tea on the ABCG8 gene expression of peripheral blood mononuclear cell (PBMC) and the atherogenic ratio ...
متن کاملEvaluation the effect of analog curcumin on the display and expression of SIRT1 and FAS genes in HepG2 fatty cells.
Abstract: Background: Non-alcoholic fatty liver is a disease that will lead to liver cirrhosis if not treated. Curcumin is the active substance of the rhizome of the turmeric plant, which has antioxidant, anti-inflammatory, antimicrobial, etc. properties. In the present study, the effects of curcumin analogue on the expression of SIRT1 and FAS genes and the accumulation of triglycerides in f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
دوره 44 7 شماره
صفحات -
تاریخ انتشار 2006